How long Covid-19 will last in the world
Preparing for the Future — Nanobodies for Covid-19? Despite the emergency use authorization issued by the FDA for antibody drugs, only a small proportion of the nation’s supply has been used.
Many had hoped that monoclonal antibody drugs would provide an important stopgap to the coronavirus disease 2019 (Covid-19) pandemic by limiting severe disease and thus the number of hospitalizations until safe and effective vaccines could be approved.1 Despite the emergency use authorization issued by the Food and Drug Administration (FDA) for antibody drugs on the basis of their ability to reduce viremia in mildly and moderately ill patients with Covid-19, only a small proportion of the nation’s supply has been used.
Myriad challenges include the therapeutic window (these drugs are more effective when administered during the first 4 to 7 days in the course of illness), the sheer number of patients during a pandemic surge and the relative paucity of infusion centers and medical staff professionals, and the emergence of mutations that affect the spike protein, which could lead to increased transmissibility and the potential for resistance to neutralization by antibodies.2 Therefore, new therapies that are effective against variants and offer an alternative to intravenously administered antibody drugs are highly desired.
A study by Koenig and colleagues3 on camelid-derived, single-domain antibodies (or nanobodies) is therefore timely. The researchers immunized alpacas and llamas with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein and identified nanobodies that specifically bind to the receptor-binding domain of the virus. They characterized four neutralizing nanobodies (labeled E, U, V, and W) structurally and functionally with multiple in vitro assays. Three of the nanobodies (U, V, and W) recognize a common epitope located near the threefold axis of the prefusion trimeric spike, whereas nanobody E recognizes the extended loop (residues R466 through P491) overlapping the receptor-binding domain (Figure 1C).
The nanobodies bound the receptor-binding domain of the virus with an equilibrium dissociation constant of between 2 and 22 nmol and neutralized SARS-CoV-2 infection by 50% in a plaque-reduction assay at concentrations ranging from 48 to 185 nmol, results similar to those achieved with monoclonal antibodies.5 In contrast to the V nanobody, nanobodies E, U, and W have the potential to prevent SARS-CoV-2 from binding angiotensin-converting enzyme 2 (ACE2) on host cells, in agreement with the location of the epitopes to which they bind and their mode of engagement with the receptor-binding domain. The nanobodies neutralize the virus by inducing a premature structural transition from a prefusion conformation to an irreversible postfusion conformation, the latter of which is incapable of binding ACE2 and thus incapable of triggering membrane fusion.
The authors then made biparatopic nanobodies (i.e., nanobodies that have two antigen-binding sites in one molecule) by fusing nanobodies that targeted distinct epitope regions (e.g., E+V, V+E, E+W, and W+E). Using cryoelectron microscopy, they showed that the most potent biparatopic nanobody (V+E) binds to all three spike proteins of the trimer (nanobody-to-trimer, 1:3 stoichiometry) with all the receptor-binding domains in the “up” conformation, indicating that the binding of nanobodies stabilizes the receptor-binding domain and prevents up–down motion, most likely contributing to proteolytic cleavage of the spike and premature transition to an irreversible postfusion conformation. The V+E biparatopic nanobody neutralized SARS-CoV-2 infection at a dilution 62 times greater than that achieved by the individual nanobodies, possibly because of the improved avidity to the spike protein (an affinity that is at least 22 times greater than that of individual nanobodies).4
While passaging a chimeric virus in Vero E6 cells in the presence of nanobodies E, U, V, and W, but not in the presence of the biparatopic (V+E or E+V) nanobodies, the authors found escape variants that had mutations within the epitope regions. This observation highlights the advantage of simultaneously targeting more than one vulnerable epitope. Of note, the footprint of the V nanobody does not include amino acids 417, 484, and 501 of the spike protein (Figure 1C), which are changed in the strains recently identified in Britain, South Africa, and Brazil, suggesting that the biparatope antibody V+E (or E+V) would be effective against these antigenic variants. The epitope recognized by nanobody V is relatively more constrained than the E epitope (which includes residues E484 and N501), meaning it is less likely to tolerate changes caused by mutation. Therefore, mutations that arise in the part of the S gene that encodes this region (i.e., the region of the spike to which the V nanobody binds) are less likely to survive selection. JJJ
Koenig et al. have contributed to the growing number of studies that have isolated nanobodies against SARS-CoV-2. Owing to the relatively small size of nanobodies, they have favorable biophysical properties and are cheaper to produce than standard monoclonal antibodies. Their small size and their long, heavy-chain complementarity-determining regions enable them to target concave epitopes such as the receptor-binding site of the spike protein.
Nanobodies can be made with the use of prokaryotic or eukaryotic expression systems because they lack the glycan-harboring Fc domain, making them easier to manufacture than standard monoclonal antibodies. The absence of an Fc region eliminates the risk of antibody-dependent enhancement of infection, but it also shortens the half-life, which could plausibly be addressed through attachment to or amalgamation with polyethylene glycol or human serum albumin. Moreover, nanobodies can be nebulized and delivered straight to the lungs of a patient with Covid-19 with an inhaler, thus presenting a better logistic alternative to intravenously administered antibodies. Aerosol formulation of nanobodies has shown promising nonclinical results.
Although nanobodies are under clinical investigation for use in a wide range of diseases from cancer to infectious diseases, it was the approval of caplacizumab (an anti–von Willebrand factor bivalent nanobody) by the European Medicines Agency and the FDA for the treatment of thrombotic thrombocytopenic purpura and thrombosis that marked the foray of nanobodies into clinical medicine. The format of the biparatopic nanobody V+E engineered by Koenig et al., although distinct from that of a conventional nanobody, is similar to that of the FDA-approved single-chain, variable fragment–based bispecific antibody blinatumomab (Figure 2).
All things considered, the available structural and clinical data suggest that the biparatopic antibody could potentially offer a better alternative to conventional monoclonal antibodies for the treatment of Covid-19. Recently, experts representing various organizations including regulatory bodies, academia, and pharmaceutical and biotechnology companies have made a call to develop small-molecule drugs that inhibit the machinery that the virus uses to replicate. Such agents are convenient to administer and insensitive to viral mutations. The biparatopic antibody, when formulated for aerosol or subcutaneous administration, will lend those benefits just as effectively.
Most health experts say Covid will be endemic, meaning it will be with us forever There’s no track record of infectious diseases being completely eradicated, and everything about COVID-19 shows that it will be no different. There are numerous reasons for this.
Many health experts say Covid is going to be endemic. www.yahoo.com/… As CBS News explains: “public health experts say… COVID-19 is never going to end. …researchers say there’s simply no track record of infectious diseases being completely eradicated, and everything about COVID-19 shows that it will be no different… Scientists say all of this makes the virus essentially impossible to control.” www.cbsnews.com/…
The Mayo Clinic’s vaccine chief said: “There is no eradication at this point, it’s off the table. The only thing we can talk about is control. amp.usatoday.com/…
The chief medical officer of BioNTech said Covid booster shots will be “necessary” because “it is the nature of immune responses that … they spike and stay for a time, but with time immune responses wane. We see this in the induced and the natural immune response against [Covid], we see this waning of immune responses.” www.cnbc.com/…
Moderna’s CEO said, “the level of antibodies [is] going to go down, that is normal and natural.” He said boosters “are going to be required” and will “be really important to keep the country safe and open.” www.foxnews.com/…
Novavax’s head of R & D agrees: “Everyone will need to be boosted. This is a viral respiratory disease, and we know from the flu that immunity from an infection is good for maybe 12 months, maybe 18 months, and after that people become susceptible again. After that, we’re going to have to boost.” www.politico.com/…
Another reason boosters are needed is mutations. In a March survey of 77 health experts, two-thirds said within a year Covid will mutate enough to make most vaccines ineffective. 18.2% think it may take longer. Since then, variants have gotten much worse, including many that show serious resistance to vaccines. reliefweb.int/…
Tests by 41 researchers indicate that variants escaping vaccines “will be inevitable.” Many richer countries plan to give variant booster shots in 2022 before half of the world even gets their first dose. www.nature.com/…
As a result, a leading market research firm (IQVIA Institute for Human Data Science) says the Covid vaccine market will total $157B by 2025, an average of $31.4B per year. www.reuters.com/…
Also, I think IQVIA is being conservative and that revenues will be higher than $157B. It reports doses in 2021 cost $22 per dose, but it assumes they will be only $9 per dose in 2023, $7 in 2024 and $5 in 2025. Those would be massive drops of 59%, 68% and 77%. I think prices will be much higher because: a) Most OWS companies have repeatedly said they’re going to raise their prices, not the other way around. b) the adenovirus vaccines aren’t suitable as boosters, which you can see here. That greatly reduces competition. c) The RNA vaccines, which are a major part of the market, can’t be profitably made at $5. d) Lots of flu vaccines exist, yet they are still $27 to $69 per dose.
Also, studies have found that about 20% of people with asymptomatic Covid get Long Covid, and a much higher % of people with mild Covid get Long Covid, which can destroy your quality of life for the rest of their life. Covid gets into nearly all organs of the human body. I don’t think that people are going to just focus on hospitalization at all. The only way to keep it under control is vaccinations.
Preparing for the Future — Nanobodies for Covid-19? Despite the emergency use authorization issued by the FDA for antibody drugs, only a small proportion of the nation’s supply has been used.
Despite the emergency use authorization issued by the FDA for antibody drugs, only a small proportion of the nation’s supply has been used.
Many had hoped that monoclonal antibody drugs would provide an important stopgap to the coronavirus disease 2019 (Covid-19) pandemic by limiting severe disease and thus the number of hospitalizations until safe and effective vaccines could be approved.1 Despite the emergency use authorization issued by the Food and Drug Administration (FDA) for antibody drugs on the basis of their ability to reduce viremia in mildly and moderately ill patients with Covid-19, only a small proportion of the nation’s supply has been used.
Myriad challenges include the therapeutic window (these drugs are more effective when administered during the first 4 to 7 days in the course of illness), the sheer number of patients during a pandemic surge and the relative paucity of infusion centers and medical staff professionals, and the emergence of mutations that affect the spike protein, which could lead to increased transmissibility and the potential for resistance to neutralization by antibodies.2 Therefore, new therapies that are effective against variants and offer an alternative to intravenously administered antibody drugs are highly desired.
A study by Koenig and colleagues3 on camelid-derived, single-domain antibodies (or nanobodies) is therefore timely. The researchers immunized alpacas and llamas with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein and identified nanobodies that specifically bind to the receptor-binding domain of the virus. They characterized four neutralizing nanobodies (labeled E, U, V, and W) structurally and functionally with multiple in vitro assays. Three of the nanobodies (U, V, and W) recognize a common epitope located near the threefold axis of the prefusion trimeric spike, whereas nanobody E recognizes the extended loop (residues R466 through P491) overlapping the receptor-binding domain (Figure 1C).
The nanobodies bound the receptor-binding domain of the virus with an equilibrium dissociation constant of between 2 and 22 nmol and neutralized SARS-CoV-2 infection by 50% in a plaque-reduction assay at concentrations ranging from 48 to 185 nmol, results similar to those achieved with monoclonal antibodies.5 In contrast to the V nanobody, nanobodies E, U, and W have the potential to prevent SARS-CoV-2 from binding angiotensin-converting enzyme 2 (ACE2) on host cells, in agreement with the location of the epitopes to which they bind and their mode of engagement with the receptor-binding domain. The nanobodies neutralize the virus by inducing a premature structural transition from a prefusion conformation to an irreversible postfusion conformation, the latter of which is incapable of binding ACE2 and thus incapable of triggering membrane fusion.
The authors then made biparatopic nanobodies (i.e., nanobodies that have two antigen-binding sites in one molecule) by fusing nanobodies that targeted distinct epitope regions (e.g., E+V, V+E, E+W, and W+E). Using cryoelectron microscopy, they showed that the most potent biparatopic nanobody (V+E) binds to all three spike proteins of the trimer (nanobody-to-trimer, 1:3 stoichiometry) with all the receptor-binding domains in the “up” conformation, indicating that the binding of nanobodies stabilizes the receptor-binding domain and prevents up–down motion, most likely contributing to proteolytic cleavage of the spike and premature transition to an irreversible postfusion conformation. The V+E biparatopic nanobody neutralized SARS-CoV-2 infection at a dilution 62 times greater than that achieved by the individual nanobodies, possibly because of the improved avidity to the spike protein (an affinity that is at least 22 times greater than that of individual nanobodies).4
While passaging a chimeric virus in Vero E6 cells in the presence of nanobodies E, U, V, and W, but not in the presence of the biparatopic (V+E or E+V) nanobodies, the authors found escape variants that had mutations within the epitope regions. This observation highlights the advantage of simultaneously targeting more than one vulnerable epitope. Of note, the footprint of the V nanobody does not include amino acids 417, 484, and 501 of the spike protein (Figure 1C), which are changed in the strains recently identified in Britain, South Africa, and Brazil, suggesting that the biparatope antibody V+E (or E+V) would be effective against these antigenic variants. The epitope recognized by nanobody V is relatively more constrained than the E epitope (which includes residues E484 and N501), meaning it is less likely to tolerate changes caused by mutation. Therefore, mutations that arise in the part of the S gene that encodes this region (i.e., the region of the spike to which the V nanobody binds) are less likely to survive selection. JJJ
Koenig et al. have contributed to the growing number of studies that have isolated nanobodies against SARS-CoV-2. Owing to the relatively small size of nanobodies, they have favorable biophysical properties and are cheaper to produce than standard monoclonal antibodies. Their small size and their long, heavy-chain complementarity-determining regions enable them to target concave epitopes such as the receptor-binding site of the spike protein.
Nanobodies can be made with the use of prokaryotic or eukaryotic expression systems because they lack the glycan-harboring Fc domain, making them easier to manufacture than standard monoclonal antibodies. The absence of an Fc region eliminates the risk of antibody-dependent enhancement of infection, but it also shortens the half-life, which could plausibly be addressed through attachment to or amalgamation with polyethylene glycol or human serum albumin. Moreover, nanobodies can be nebulized and delivered straight to the lungs of a patient with Covid-19 with an inhaler, thus presenting a better logistic alternative to intravenously administered antibodies. Aerosol formulation of nanobodies has shown promising nonclinical results.
Although nanobodies are under clinical investigation for use in a wide range of diseases from cancer to infectious diseases, it was the approval of caplacizumab (an anti–von Willebrand factor bivalent nanobody) by the European Medicines Agency and the FDA for the treatment of thrombotic thrombocytopenic purpura and thrombosis that marked the foray of nanobodies into clinical medicine. The format of the biparatopic nanobody V+E engineered by Koenig et al., although distinct from that of a conventional nanobody, is similar to that of the FDA-approved single-chain, variable fragment–based bispecific antibody blinatumomab (Figure 2).
All things considered, the available structural and clinical data suggest that the biparatopic antibody could potentially offer a better alternative to conventional monoclonal antibodies for the treatment of Covid-19. Recently, experts representing various organizations including regulatory bodies, academia, and pharmaceutical and biotechnology companies have made a call to develop small-molecule drugs that inhibit the machinery that the virus uses to replicate. Such agents are convenient to administer and insensitive to viral mutations. The biparatopic antibody, when formulated for aerosol or subcutaneous administration, will lend those benefits just as effectively.
Most health experts say Covid will be endemic, meaning it will be with us forever There’s no track record of infectious diseases being completely eradicated, and everything about COVID-19 shows that it will be no different. There are numerous reasons for this.
There’s no track record of infectious diseases being completely eradicated, and everything about COVID-19 shows that it will be no different. There are numerous reasons for this.
Many health experts say Covid is going to be endemic. www.yahoo.com/… As CBS News explains: “public health experts say… COVID-19 is never going to end. …researchers say there’s simply no track record of infectious diseases being completely eradicated, and everything about COVID-19 shows that it will be no different… Scientists say all of this makes the virus essentially impossible to control.” www.cbsnews.com/…
The Mayo Clinic’s vaccine chief said: “There is no eradication at this point, it’s off the table. The only thing we can talk about is control. amp.usatoday.com/…
The chief medical officer of BioNTech said Covid booster shots will be “necessary” because “it is the nature of immune responses that … they spike and stay for a time, but with time immune responses wane. We see this in the induced and the natural immune response against [Covid], we see this waning of immune responses.” www.cnbc.com/…
Moderna’s CEO said, “the level of antibodies [is] going to go down, that is normal and natural.” He said boosters “are going to be required” and will “be really important to keep the country safe and open.” www.foxnews.com/…
Novavax’s head of R & D agrees: “Everyone will need to be boosted. This is a viral respiratory disease, and we know from the flu that immunity from an infection is good for maybe 12 months, maybe 18 months, and after that people become susceptible again. After that, we’re going to have to boost.” www.politico.com/…
Another reason boosters are needed is mutations. In a March survey of 77 health experts, two-thirds said within a year Covid will mutate enough to make most vaccines ineffective. 18.2% think it may take longer. Since then, variants have gotten much worse, including many that show serious resistance to vaccines. reliefweb.int/…
Tests by 41 researchers indicate that variants escaping vaccines “will be inevitable.” Many richer countries plan to give variant booster shots in 2022 before half of the world even gets their first dose. www.nature.com/…
As a result, a leading market research firm (IQVIA Institute for Human Data Science) says the Covid vaccine market will total $157B by 2025, an average of $31.4B per year. www.reuters.com/…
Also, I think IQVIA is being conservative and that revenues will be higher than $157B. It reports doses in 2021 cost $22 per dose, but it assumes they will be only $9 per dose in 2023, $7 in 2024 and $5 in 2025. Those would be massive drops of 59%, 68% and 77%. I think prices will be much higher because: a) Most OWS companies have repeatedly said they’re going to raise their prices, not the other way around. b) the adenovirus vaccines aren’t suitable as boosters, which you can see here. That greatly reduces competition. c) The RNA vaccines, which are a major part of the market, can’t be profitably made at $5. d) Lots of flu vaccines exist, yet they are still $27 to $69 per dose.
Also, studies have found that about 20% of people with asymptomatic Covid get Long Covid, and a much higher % of people with mild Covid get Long Covid, which can destroy your quality of life for the rest of their life. Covid gets into nearly all organs of the human body. I don’t think that people are going to just focus on hospitalization at all. The only way to keep it under control is vaccinations.